光語生物微囊藻毒素試劑盒常見20個問題

　　20 Common Questions About Lightary Microcystin ELISA Kit

　　微囊藻毒素檢測試劑盒作為一種快速檢測工具，被廣泛應用于水質(zhì)監(jiān)測、食品安全等領域。

　　As a rapid detection tool,microcystin test kits are widely used in water quality monitoring,food safety and other fields.

　　1.為什么選擇光語生物微囊藻毒素檢測試劑盒？

　　光語生物微囊藻毒素檢測試劑盒是聯(lián)合國內(nèi)外專業(yè)團隊獨創(chuàng)技術，檢測靈敏度達0.1μg/L，能精準捕捉微囊藻毒素污染。市場上的大型檢測儀器價格昂貴，操作難，但光語生物微囊藻毒素檢測試劑盒不用專業(yè)技術，操作簡單，速度快，成本只有大型儀器的幾十分之一，特別適合大量檢測。

　　1.Why choose Lightary Microcystin ELISA Kit?

　　The Lightary Microcystin ELISA Kit is independently developed by a professional team integrating domestic and international expertise,with a detection sensitivity of 0.1μg/L to accurately capture microcystin contamination.Unlike expensive and complex large-scale detection instruments on the market,this kit requires no specialized technical skills,features simple operation and fast detection speed,and costs only 1/10th to 1/50th of traditional instruments,making it particularly suitable for high-throughput screening.

　　2.這個試劑盒價格是多少？貴不貴？

　　光語生物微囊藻毒素試劑盒全國統(tǒng)一價3500元，有96個反應單元，可供42個樣品檢測，每個樣品檢測費用少于50元，多買享有折扣，與進口試劑盒相比較（已超過6000元/盒），質(zhì)量保障，檢測靈敏，價格適中，且質(zhì)量有保障。

　　2．What is the price of this kit?Is it expensive?

　　The national unified price of the microcystin kit is 3,500 yuan,containing 96 reaction units that can be used for detecting 42 samples,with the detection cost per sample being less than 50 CNY.Discounts are available for bulk purchases.Compared with imported kits(which cost over 6,000 CNY per kit),it ensures quality,has sensitive detection,and a moderate price.

　　3.那什么是微囊藻毒素，為什么要檢測它呢？

　　微囊藻毒素（MCs）是由藍藻產(chǎn)生的一類環(huán)狀七肽毒素，常見于被生活、農(nóng)業(yè)、工業(yè)等廢水污染的水體中。它是水里的“有毒小炸彈”，一旦飲用了含毒素的水，可能會傷肝、致癌，極性則表現(xiàn)為拉肚子、甚至死亡，對人和動物都很危險，因此我們需要使用專業(yè)的試劑盒對它進行檢測，保證我們的水體安全。

　　3.What is microcystin,and why is it important to detect it?

　　Microcystins(MCs)are a class of cyclic heptapeptide toxins produced by cyanobacteria(blue-green algae),commonly found in water bodies polluted by domestic,agricultural,and industrial wastewater.Referred to as”toxic bombs”in water,consumption of toxin-contaminated water can cause liver damage,carcinogenic risks,and acute symptoms like diarrhea or even death,posing severe dangers to both humans and animals.Professional test kits are therefore essential for detecting MCs to ensure water safety.

　　4.光語生物微囊藻毒素檢測試劑盒檢測原理復雜嗎？

　　該試劑盒采用酶聯(lián)免疫吸附試驗（ELISA），通過抗原-抗體特異性結(jié)合，酶標二抗催化底物顯色。簡單的說，就像鑰匙找鎖孔！毒素是“鑰匙”，試劑盒里的抗體是“鎖孔”，它們一配對，再加上特殊試劑，就能用顏色深淺判斷毒素有多少，簡單好懂，易上手。

　　4.Is the detection principle complex?

　　The kit uses an Enzyme-Linked Immunosorbent Assay(ELISA),which relies on specific binding between antigens and antibodies,with enzyme-labeled secondary antibodies catalyzing substrate color development.Simplistically,it is analogous to a”key-lock”mechanism:the toxin acts as the”key,”and the antibody in the kit serves as the”lock.”Once they pair,the addition of special reagents allows determination of toxin concentration by the intensity of color developed—a straightforward,easy-to-understand principle that is simple to use..

　　5光語生物微囊藻試劑盒適用群體有哪些？

　　該試劑盒應用范圍廣，湖泊、河流、池塘等水體均能檢測，通常適用但不僅限于以下群體：

　　1）高校、研究所等科研機構(gòu)，用于研究湖泊、水庫、河流等水體中微囊藻毒素分布。

　　2）水利部門、水廠等單位用于水源地定期檢測，如某湖泊微囊藻毒素臨近限值時，水廠啟動應急處理技術。

　　3）生態(tài)環(huán)境部門用于水體藍藻水華藻毒素快速篩查，出具微囊藻毒素濃度數(shù)據(jù)，助力下游供水預警。

　　4）水產(chǎn)養(yǎng)殖戶用于自主檢測池塘微囊藻毒素濃度，提前預防魚蝦肝損傷，避免因毒素超標導致重大經(jīng)濟損失。

　　5.Which groups is the Lightary Microcystin ELISA Kit suitable for?

　　The kit has a wide range of applications and can detect water bodies such as lakes,rivers,and ponds.It is typically suitable for,but not limited to,the following groups:

　　1)Scientific research institutions(e.g.,universities,research institutes)for studying the distribution of microcystins in water bodies such as lakes,reservoirs,and rivers.

　　2)Water conservancy departments and water treatment plants for regular testing of water sources.For example,when microcystin levels in a lake approach regulatory limits,water plants can initiate emergency treatment technologies.

　　3)Ecological environment departmentsfor rapid screening of cyanotoxin levels during cyanobacterial blooms in water bodies,providing microcystin concentration data to support downstream water supply warnings.

　　4)Aquaculture farmersfor independent testing of microcystin concentrations in ponds,enabling early prevention of liver damage in fish and shrimp and avoiding significant economic losses caused by toxin exceedances.

　　6.光語生物微囊藻毒素試劑盒檢測一次要多久，需要具備哪些條件，不會操作怎么辦？

　　該試劑盒檢測時間通常在2-4小時，對于一般的水體毒素檢測只需要準備一臺酶標儀，對于細胞內(nèi)或組織毒素檢測可能還需要破碎儀或超聲破碎裝置進行樣品預處理。對于操作問題，我們配備有超詳細的操作手冊和視頻，跟著“加樣、等一等、看顏色”三個步驟，小白也能輕松上手。

　　6.How long does a test with the Lightary Microcystin Detection Kit take,what equipment is required,and what if I am unsure about the operation??

　　The detection time of this kit typically ranges from 2 to 4 hours.For general water toxin detection,only a microplate reader is required.For intracellular or tissue toxin detection,sample pretreatment may also require a homogenizer or ultrasonic crushing device.Regarding operation,we provide extremely detailed operation manuals and videos.Following the three steps of”sample loading,incubation,and color observation”,even beginners can easily get started.

　　7.使用該試劑盒檢測微囊藻毒素時要注意些什么？

　　1）測試前，戴上手套，把試劑和樣本放至常溫，別讓陽光直射，保持桌面干凈。

　　2）樣本盡量破碎完全，保證毒素充分釋放，保證數(shù)據(jù)準確。

　　3）拍板時注意避免孔位之間交叉污染，影響數(shù)據(jù)準確性。

　　4）試劑盒里有“檢查官”——對照樣本和標準曲線，測得數(shù)據(jù)要控制在標曲范圍內(nèi)才可信。

　　5）標準曲線需與樣品同步檢測，避免因試劑批次、溫度或儀器差異導致誤差。

　　6）對于高濃度樣本，檢測前把樣品稀釋10倍、100倍再測，最后乘上稀釋倍數(shù)，輕松解決。

　　7）用完的試劑盒不能亂扔！分類打包好，交給專門處理實驗室垃圾的公司，保護環(huán)境人人有責。

　　8）沒用完的試劑盒放置2-8℃冰箱，在有效內(nèi)均可使用。

　　7.What precautions should be taken when using the kit to detect microcystins??

　　?Test Preparation:Wear gloves before testing.Equilibrate reagents and samples to room temperature,avoid direct sunlight,and ensure a clean workbench.

　　?Sample Disruption:Fully disrupt samples(e.g.,via homogenization or sonication)to ensure complete toxin release,which is critical for accurate data.

　　?Cross-Contamination Avoidance:Take care to prevent cross-contamination between wells during plate washing or pipetting,as this may compromise data accuracy.

　　?The standard curve must be tested synchronously with the samples to avoid errors caused by reagent batch variations,temperature fluctuations,or instrument discrepancies.

　　?Quality Control:The kit includes”quality control officers”—control samples and a standard curve.Test results must fall within the standard curve range to be valid(refer to ISO 17025:2017 guidelines for calibration validation).

　　?High-Concentration Samples:For samples with suspected high toxin levels,dilute them 10×or 100×before testing,then multiply the result by the dilution factor to obtain the actual concentration(following EPA method 10210 for cyanotoxin analysis).

　　?Waste Disposal:Do not discard used kits casually.Classify and package waste according to biohazard protocols,and entrust it to licensed laboratory waste disposal companies(in compliance with WHO guidelines for biomedical waste management).

　　?Storage:Unused kits should be stored at 2–8°C.Ensure they are used within the expiration date stated on the package.

　　8.該試劑盒檢測數(shù)據(jù)能用在報告里嗎？

　　當然！我們有CMA認證和ISO 17025標準認證，政府檢查、寫論文都能用，權威有保障。

　　8.Can the data be used in reports?

　　Certainly!The kit has CMA certification and complies with ISO 17025 standards,making the data usable for government inspections,research papers,and other authoritative purposes with guaranteed credibility.

　　9.檢測過程中，發(fā)現(xiàn)水樣太臟測應該如何處理樣品？

　　天然水體中由于水體參雜各種污染物，雜質(zhì)，導致測定困難。因此在檢測前，我們可以用過濾膜過濾樣品，或者在條件允許的情況下進行離心、活性炭吸附，去掉雜質(zhì)就能測了。

　　9.What should be done to process dirty water samples during testing?

　　Natural water bodies often contain various pollutants and impurities,which may interfere with detection.To address this:Filter the sample using a membrane filter(e.g.,0.45μm nitrocellulose membrane)before testing.If equipment is available,centrifuge the sample(e.g.,5,000 rpm for 10 minutes)to pellet solids.Alternatively,use activated carbon adsorption to remove organic contaminants.These preprocessing steps help eliminate impurities and ensure accurate measurement results.Detailed methods are provided in the kit’s instruction manual.

　　10.試劑盒的標準曲線有效期是多久？是否需要每次檢測都重新繪制？

　　每次檢測需重新繪制并同步運行標準曲線，確保曲線線性關系（R2≥0.99），以保證定量準確性。

　　10.How long is the standard curve valid?Does it need to be redrawn for each test?

　　A standard curve must be redrawn and run synchronously for each test to ensure a linear relationship(R2≥0.99),thereby guaranteeing quantitative accuracy..

　　11.如何確保檢測結(jié)果的準確性？

　　重復檢測：對同一樣本進行2–3次平行試驗，排除操作誤差。

　　梯度稀釋驗證：若結(jié)果超出標曲上限，需進一步稀釋樣本確認濃度。

　　交叉污染排查：檢查加樣槍、微孔板是否清潔，避免孔間污染導致假陽性。

　　11.What should be done if abnormal results are obtained?

　　Repeat the test 2–3 times for parallel verification.If results exceed the standard curve range,further dilute the sample to confirm concentration and check for cross-contamination.

　　12.試劑盒能否檢測微囊藻毒素的所有亞型？

　　該主要針對微囊藻毒素-LR（MC-LR）設計，但對MC-RR、MC-YR等常見亞型，會有交叉反應，（如對MC-RR的交叉反應為70.4%，對MC-YR交叉反應為51.3%），因此采樣該試劑盒檢測的是微囊藻毒素的總毒素，并非僅MC-LR。12.Can the kit detect all subtypes of microcystins?

　　This kit is primarily designed for microcystin-LR(MC-LR),but it may exhibit cross-reactivity with common subtypes such as MC-RR and MC-YR(e.g.,cross-reactivity of 70.4%with MC-RR and 51.3%with MC-YR).Therefore,testing with this kit measures the total microcystin toxin content**rather than MC-LR alone.

　　13.孕婦或敏感人群接觸試劑盒試劑是否有風險？

　　該試劑需避免直接接觸皮膚或黏膜。操作時建議佩戴手套和護目鏡，若不慎接觸，立即用清水沖洗，無需特殊防護措施。

　　13.Is there any risk for pregnant women or sensitive groups to handle kit reagents?

　　Reagents contain horseradish peroxidase(HRP)and trace preservatives.Avoid direct skin/mucus contact.Wear gloves and goggles;rinse with water if exposed.

　　14.夏季高溫環(huán)境下如何儲存和運輸試劑盒？

　　儲存：未開封試劑盒需始終置于2–8℃冰箱，避免凍結(jié)（凍結(jié)會破壞抗體活性）。

　　運輸：采用冷鏈保溫箱（配備冰袋），確保運輸溫度維持在2–8℃，避免暴曬或高溫。

　　14.How to store and transport the kit in high-temperature summer environments?

　　Store unopened kits at 2–8℃to prevent freezing(which damages antibody activity).Use cold-chain insulation boxes with ice packs for transportation to maintain 2–8℃.

　　15.能否用該試劑盒檢測土壤或沉積物中的微囊藻毒素？

　　可以。需先進行毒素提取：稱取5–10 g樣本，超聲提取30分鐘，離心后取上清液檢測。提取效率可能受土壤有機質(zhì)影響，建議通過加標回收試驗驗證。

　　15.Can the kit detect microcystins in soil or sediment?

　　Sample extraction is required:weigh 5–10 g of sample,extract with methanol/water(1:1)via sonication for 30 min,centrifuge,and test the supernatant.Organic matter may affect extraction efficiency.

　　16.試劑盒與其他品牌產(chǎn)品的兼容性如何？

　　不建議混合使用不同品牌的試劑（如酶標二抗、底物），可能因抗體來源差異導致結(jié)果偏差。若需更換試劑，需重新驗證標準曲線和檢測流程。

　　16.Is the kit compatible with products from other brands?

　　Mixing reagents from different brands(e.g.,enzyme-labeled secondary antibodies,substrates)is not recommended due to potential antibody-source variations.Re-validate the standard curve if reagents are changed.

　　17.檢測后的微孔板能否重復使用？

　　不可重復使用：微孔板表面經(jīng)包被抗體處理，重復使用會導致抗體脫落和交叉污染，影響結(jié)果準確性。

　　17.Can the microplate be reused after testing?

　　No.The microplate is coated with antibodies,and reuse may cause antibody detachment and cross-contamination.

　　18.試劑盒是否提供陰/陽性對照樣本？

　　本試劑盒使用時可使用無菌純水作為陰性對照，標準樣品作為陽性對照（如1μg/L MC-LR），用于驗證試劑有效性和操作正確性。

　　18.Does the kit include negative/positive control samples?

　　When using this kit,sterile pure water can be used as the negative control,and standard samples(such as 1μg/L MC-LR)can be used as the positive control to verify the effectiveness of the reagents and the correctness of the operation.

　　19.如何通過試劑盒測定數(shù)據(jù)判斷水質(zhì)安全，水體微囊藻毒素安全閾值是多少？

　　中國國家標準（GB 5749-2022《生活飲用水衛(wèi)生標準》）規(guī)定：微囊藻毒素-LR（MC-LR）的安全限值為1.0μg/L（適用于飲用水源地），高效液相色譜法和酶聯(lián)免疫吸附法的檢出限：均為0.1μg/L，客戶需根據(jù)樣品是否稀釋來計算最終毒素濃度，從而判斷水體毒素是否超標。

　　19.How to determine water safety based on the kit’s test data and what is the safety threshold for microcystins in water?

　　According to the Chinese National Standard GB 5749-2022 Standards for Drinking Water Quality:The safety limit for microcystin-LR(MC-LR)in drinking water sources is 1.0μg/L.The detection limit for both high-performance liquid chromatography(HPLC)and enzyme-linked immunosorbent assay(ELISA)is 0.1μg/L.

　　Data interpretation and judgment steps:

　　Calculate the actual toxin concentration by multiplying the measured value with the dilution factor(if the sample was diluted before testing).

　　Compare the final concentration with the national standard:

　　?<0.1μg/L:Undetected,meeting safety requirements.

　　?≥0.1μg/L and<1.0μg/L:Requires enhanced monitoring and pollution source investigation.

　　?≥1.0μg/L:Exceeds the safety threshold,necessitating immediate emergency measures(e.g.,activated carbon treatment,suspension of water intake).

　　?

　　20.微囊藻毒素檢測試劑盒標標準曲線如何理解，如何利用準曲線計算實際測量微囊藻毒素值？

　　該試劑盒標準曲線為標準樣品B0值（每個樣品OD值與陰性對照平均值之間的比值）與其對應濃度數(shù)值對數(shù)之間的關系曲線，因此測得對應OD值后，求出B0值，再利用曲線求出濃度對數(shù)（以10為底，濃度的對數(shù)），換算出來即可，注意單位（詳細步驟，見視頻講解）。

　　利用標準曲線計算實際濃度的步驟

　　1.測定OD值：

　　Measure the OD(optical density)values of all samples(including standards,negatives,and test samples)using a microplate reader at the specified wavelength(e.g.,450 nm).

　　2.計算B0值：

　　o計算陰性對照（Negative Control,NC）的平均OD值，記為ODNC-average?。

　　o對于每個標準品或待測樣品，其B0值計算公式為：

　　（本試劑盒無需×100%）

　　3.查標準曲線或代入回歸方程：

　　o在標準曲線上找到與樣品B0值對應的濃度對數(shù)值（以10為底，即log10?(concentration)）。

　　o若已知曲線的回歸方程如log10(concentration)=a×B0 value+b，代入樣品的B0值計算對數(shù)值。

　　4.換算為實際濃度：

　　o對求得的濃度對數(shù)值取反對數(shù)（以10為底），得到樣品的實際濃度（單位：μg/L）。Concentration=10log10?(concentration)

　　5.稀釋倍數(shù)校正（如有）：

　　If the sample was diluted before testing,multiply the calculated concentration by the dilution factor to obtain the final result.

　　示例說明

　　?已知標準曲線方程：log10?(concentration)=?0.02×B0 value+2

　　?測得某樣品的B0值：50%

　　?計算過程：log10?(concentration)=?0.02×50+2=1?Concentration=101=10μg/L

　　注意事項

　　?曲線線性范圍：確保樣品的B0值落在標準曲線的線性區(qū)間內(nèi)（通常為標準品濃度范圍的50%–150%），超出范圍需稀釋后重新檢測。

　　?單位一致性：標準曲線的濃度單位需與檢測目標一致（如μg/L），避免換算錯誤。

　　?操作規(guī)范性：標準曲線需與樣品同步檢測，避免因試劑批次、溫度或儀器差異導致誤差（詳細操作見試劑盒配套視頻講解）。

　　20.How to understand the standard curve of the microcystin detection kit and calculate the actual microcystin concentration using the curve?

　　The standard curve of the kit represents the relationship between the B0 value of standard samples(the ratio of each sample’s OD value to the average OD value of negative controls)and the logarithm of their corresponding concentrations(base 10).This curve is established by plotting known standard concentrations against their respective B0 values,forming a logarithmic regression model to quantify unknown samples.